Table 2.
Frequency of bacterial isolates with (increased MIC)/(changes in clinical interpretation) when comparing standard test medium (Mueller Hinton broth, no albumin) vs. Mueller Hinton broth supplemented with clinically relevant levels of canine albumin.
| Percent bacterial isolates with increased MIC/changes in clinical interpretation | |||||
|---|---|---|---|---|---|
| from albumin added to the test medium | |||||
| Antibiotic | Protein binding (%) | Pseudomonas aeruginosa | Staphylococcus pseudintermedius | Streptococcus canis | All bacterial isolates |
| Amikacin | ≤10% | 0/0 | 0/0 | 0/0 | 0/0 |
| Bacitracin | Not available | 0/0 | 0/0 | 60/60 | 20/20 |
| Cefazolin | 74–86% | 0/0 | 0/0 | 0/0 | 0/0 |
| Ceftiofur | >90% | 0/0 | 0/0 | 0/0 | 0/0 |
| Chloramphenicol | 50–60% | 0/0 | 100/50 | 0/0 | 33/17 |
| Ciprofloxacin | 20–40% | 0/0 | 0/0 | 10/10 | 3/3 |
| Doxycycline | >90% | 0/0 | 60/0 | 70/0 | 43/0 |
| Erythromycin | 80–93% | 0/0 | 40/30 | 0/0 | 13/10 |
| Gentamicin | 0–30% | 0/0 | 90/30 | 100/100 | 63/43 |
| Moxifloxacin | 50% | 20/0 | 10/0 | 0/0 | 10/0 |
| Neomycin | 40% | 70/0 | 10/0 | 0/0 | 27/0 |
| Ofloxacin | 32% | 100/90 | 80/30 | 80/70 | 87/63 |
| Oxytetracycline | 20–25% | 70/80 | 10/10 | 10/0 | 30/30 |
| Polymyxin B | 92–99% | 10/0 | 0/0 | 0/0 | 3/0 |
| Ticarcillin | 45% | 100/90 | 10/10 | 0/0 | 37/33 |
| Tobramycin | <30% | 0/0 | 30/30 | 100/100 | 43/43 |
| Trimethoprim/sulfamethoxazole | 45–75% | 0/0 | 0/0 | 0/0 | 0/0 |
Percent protein binding inherent to each antibiotic is depicted in the second column for reference. MIC, minimal inhibitory concentration.