Table 1.
Typical RNA, protein, and DNA yields from the PDLO sandwich culturea
| Well format | Sample | Typical yields |
|---|---|---|
| 12-well sandwich culture | RNA | 2.5–7.5 μg |
| 6-well sandwich culture | Protein (RIPA lysis) | 100–200 μg |
| 12-well sandwich culture | DNA | 2.5–7.5 μg |
PDLOs were harvested at day 27–30 or 10–12 days after splitting.