| Day |
Additives to the media |
Stock concentration |
Final concentration |
Dilution |
|---|---|---|---|---|
| BE3 | ||||
| d13–20 (d13,17) | Nicotinamide | 1 M | 10 mM | 1:100 |
| ZnSO4 | 10 mM | 10 μM | 1:1,000 | |
| Rock inhibitor Y-27632 | 10 mM | 10 μM | 1:1,000 | |
| EGF | 50 μg/mL | 50 ng/mL | 1:1,000 | |
| FGF10 | 50 μg/mL | 50 ng/mL | 1:1,000 | |
| KGF | 50 μg/mL | 50 ng/mL | 1:1,000 | |
| MSC2530818 | 5 mM | 50 nM | 1:100,000 | |
| GFR Matrigel∗ | 100% | 5% | 1:20 | |
Prepare differentiation media at the day of medium change; day of medium change is given in brackets; d: day. ∗ Add GFR with precooled tips directly before use.