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. 2021 Dec 8;2(4):100913. doi: 10.1016/j.xpro.2021.100913
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
CXCR4 antibody (1:50),
Mouse monoclonal PE-conjugated		 Life Technologies Cat# MHCXCR404; RRID:AB_10373097
EPCAM antibody (1:33.33),
Mouse monoclonal APC-conjugated		 BD Cat# 347200; RRID:AB_400570
GATA6 (1:500),
Rabbit monoclonal unconjugated		 Cell Signaling Technology Cat# 5851;
RRID:AB_10705521
GP2 (1:5000),
Mouse monoclonal unconjugated		 MBL International Cat# D277-3;
RRID:AB_10598500
KIT antibody (1:100),
Mouse monoclonal APC-conjugated		 Thermo Cat# CD11705; RRID:AB_2536476
NKX6-1 (1:150->final conc. 1μg/mL),
Mouse monoclonal unconjugated		 Developmental Studies Hybridoma Bank (DSHB) Cat# F55A12 concentrate; RRID:AB_532379
NKX6-1 (1:35),
Mouse monoclonal APC-conjugated		 BD Cat# 563338; RRID:AB_2738144
PDX1 (1:500),
Goat polyclonal unconjugated		 R&D Cat# AF2419; RRID:AB_355257
PDX1-PE (1:35),
Mouse monoclonal unconjugated		 BD Cat# 562161; RRID:AB_10893589
SOX17 (1:500),
Goat polyclonal unconjugated		 R&D Cat# AF1924;
RRID:AB_355060
Chemicals, peptides, and recombinant proteins
Accutase Merck Cat# A6964
Activin A PeproTech Cat# 120-14;
SDS: 25-120-14
Activin A R&D Cat# 338-AC
Agarose Merck Cat# A9539
CHIR99021 Axon MedChem Cat# 1386;
CAS: 252917-06-9
Collagenase/dispase Roche Cat# 11097113001
DMEM/F12 Gibco Cat# 12634010
DMSO Merck Cat# D2650
Dorsomorphin Merck Cat# P5499;
CAS: 866405-64-3
Ethanol (EtOH) Thermo Cat# 32205
EGF human R&D Cat# 236-EG-200
Fatty acid free BSA Proliant Cat# 68700;
CAS: 9048-46-8
FGF2 R&D Cat# 233-FB
FGF10 PeproTech Cat# 100-26
FGF10 R&D Cat# 345-FG
Glucose Merck Cat# G7528
CAS: 50-99-7
Hydrochloric acid (HCL) (37%) Merck Cat# 30721-M
Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) Thermo Cat# 51500056
KGF PeproTech Cat# 100-19;
SDS: 25-100-19
L-Ascorbic acid Merck Cat# A4544
CAS: 50-81-7
L-glutamine (Glutamax) Thermo Fisher Scientific Cat# 35050038
LDN-193189 Merck Cat# SML0559;
CAS: 1062368-24-4
Matrigel Basement Membrane Matrix Growth Factor Reduced (GFR) Corning Cat# 354230
Matrigel hESC-qualified Matrix Corning Cat# 354277
MCDB131 medium Thermo Fisher Scientific Cat# 10372019
MSC2530818 Selleckchem Cat# S8387;
CAS: 1883423-59-3
mTeSR1 STEMCELL Technologies Cat# 85850
mTesR Plus STEMCELL Technologies Cat# 100-0276
Nicotinamide (NA) Merck Cat# N0636;
CAS: 98-92-0
NutriFreez D10 Sartorius Cat# 05-713
Paraformaldehyde (PFA) Merck Cat# P6148
Penicillin/Streptomycin (P/S) Merck Cat# P4333
Retinoic acid (RA) Merck Cat# R2625;
CAS: 302-79-4
ROCK inhibitor (Y-27632) Abcam ab120129;
CAS: 129830-38-2
SANT1 Merck Cat# S4572;
CAS: 304909-07-7
Sodium bicarbonate (NaHCO3) Merck Cat# S5761
CAS: 144-55-8
Sodium dihydrogen phosphate mnohydrate (NaH2PO4× H2O) Fluka 71507
Disodium hydrogen phosphate heptahydrate (Na2HPO4× 7H2O) Sigma S9390
Sodium hydroxide (NaOH) AppliChem AP131687.1211
Succrose D (+) Fluka 84100
Tissue-Tek O.C.T. compound Sakura Cat# 4583
TrypLE™ Express Enzyme, no phenol red Thermo Fisher Scientific Cat# 12604013
TrypLE™ Select Enzyme, no phenol red Thermo Fisher Scientific Cat# 12563011
Wnt3a mouse PeproTech Cat# 315-20;
SDS: 25-315-20
Zinksulfate (ZnSO4) Merck Cat# Z0251;
CAS: 7446-20-0
(-)-Indolactam V STEMCELL Technologies Cat# 72312;
CAS: 90365-57-4
Critical commercial assays
Tissue Genomic DNA Purification Mini Prep Kit Genaxxon Cat# S5378.0050
GeneJET RNA Purification Kit Thermo Cat# K0732
Experimental models: Cell lines
HUES8 hESC line (male, NIH approval number NIHhESC-10-0021) HSCI iPS Core, Harvard University, Cambridge, MA, USA hES Cell Line: HUES-8; RRID:CVCL_B207
iPSCs (male, Institutional review board of Ulm University (registration number 68/11-UBB/bal) Breunig et al. (2021) n/a
Other
Counting chamber VWR Cat# HIRS8100104
Freezing container VWR Cat# 479-3200
LSR II flow cytometer BD n/a
SuperFrost plus slides VWR Cat# 631-0108