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. 2021 Dec 14;43(8):2147–2155. doi: 10.1038/s41401-021-00818-x

Fig. 2. Biochemical properties of DPP4-WT and DPP4-V486M.

Fig. 2

a Stability comparison of WT and V486M. ∆Tm is indicated the difference between mean value of WT and V486M. Mean ± SD values are shown. Inhibitors binding activity comparison of WT (b) and V486M (c) in the absence or presence of DPP4 inhibitor omargliptin. ∆Tm is indicated the difference between mean value of DMSO control and Omarigliptin group. Mean ± SD values are shown. d, e Coomassie blue-stained reducing SDS-PAGE and nonreducing SDS-PAGE (10%) of WT and V486M. e The dimer percent statistics of WT and V486M. The percent 40.8% and 22.7%, indicates dimer percent of WT and V486M, respectively. R, NR, D, M, presents reducing SDS-PAGE, nonreducing SDS-PAGE, dimer, and monomer.