Table 2.

Enzyme kinetic constants of flap variants.

Variants	Km (μM)	Kcat (min−1)	Kcat/Km (min−1·μM−1)
WT	37.96 ± 2.03	1965.38 ± 31.70	47.77 ± 4.38
K122D	33.60 ± 3.86	477.59 ± 16.15	13.46 ± 0.70
P257A	49.55 ± 1.80	1730.80 ± 20.27	36.46 ± 1.75
D243A	43.87 ± 2.31	2415.70 ± 39.79	55.57 ± 0.55
S664P	NDa	ND	ND

Mean ± SD values are shown.

^aND indicates not detected.