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Influence of the (A and B) serum concentration, (C and D) serum source, and (E and F) insulin-like growth factor 1 (IGF-1) on the chicken intestinal organoid growth (scale bar, 200 μm). Error bars, SEM (n = 3 wells). Experiments were performed at least 3 times and a representative one was exhibited. Error bars, SEM (n = 3 wells). ∗, P ≤ 0.05. a, b Different letters on bars mean a significant difference (P ≤ 0.05). CS = chicken serum; FBS = fetal bovine serum.
