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. 2021 Sep 3;8:38–51. doi: 10.1016/j.aninu.2021.06.001

Fig. 7.

Fig. 7

Chicken crypt growth and development under different degrees of methionine deficiency. (A) Growth of chicken crypts under different treatments (scale bar, 200 μm). (B) Number of live intestinal organoids obtained with different treatments. (C to G) Comparison of relative mRNA expression of mature intestinal epithelial markers in crypts cultured for 5 d in medium with methionine or in medium additionally supplemented with 10 mmol/L cycloleucine. (H) Comparison of relative mRNA expression of MAT2A in crypts cultured for 5 d in medium with methionine or in medium additionally supplemented with 10 mmol/L cycloleucine. Experiments were performed at least 3 times and a representative one was exhibited. Error bars, SEM (n = 3 wells). a to g Different letters on bars mean a significant difference (P ≤ 0.05). ∗P < 0.05; ∗∗P < 0.01. LGR5 = leucine-rich-repeat-containing G-protein-coupled receptor 5; LYZ = lysozyme; SI = sucrase-isomaltase; MUC2 = mucin 2; CHGA = chromogranin A; MAT2A = methionine adenosyltransferase 2A.