Fig. 1.

Glycine (Gly) inhibited brefeldin A (BFA)-induced apoptosis in intestinal epithelial (IPEC-1) cells. Apoptosis were determined by flow cytometric analysis (A) and analyzed (B). Cell viability (C) was assessed by Cell Counting Kit-8 (CCK8) assay. Protein abundances of c-Caspase3, Bax, p53, Bcl-2, and Bcl-xL were analyzed by Western blot (D) and (E). Values are means ± SEM of 3 independent experiments. Means without a common letter differ, P < 0.05. FITC = fluorescein Isothiocyanate; c-caspase3 = cleaved-caspase3; Bcl-2 = B-cell lymphoma.