Fig. 2.

Glycine repressed brefeldin A (BFA) -triggered IRE1α-JNK signaling in intestinal epithelial (IPEC-1) cells. Protein abundances of ATF6α, p-eIF2α, eIF2α, p-IRE1α, IRE1α, p-JNK, JNK, and CHOP were analyzed by Western blot (A) and relative protein expression levels were normalized to GAPDH (B). Ultrastructure of rough endoplasmic reticulum under transmission electron microscope (C). Values are means ± SEM of 3 independent experiments. Relative expression of a protein without a common letter differ, P < 0.05. Scale bar: 200 nm. IRE1α = inositol-requiring enzyme 1α; JNK = c-Jun N-terminal kinase.