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Glycine alleviated brefeldin A (BFA)-induced intestinal barrier dysfunction in mTORC1-dependent manner. Transepithelial electrical resistance (TEER) (A) and paracellular permeability (B) were determined. Protein abundances (C) and localization of occludin, claudin-1, claudin-3, zonula occludens (ZO)-1, ZO-2 and ZO-3 (D) were analyzed by Western blot or immunofluorescence. Means without a common letter differ, P < 0.05. Scale bar: 50 μm.
