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. 2021 Nov 30;8:772324. doi: 10.3389/fmed.2021.772324

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Copyright © 2021 Obaíd, Camacho, Brenet, Corrales-Orovio, Carvajal, Martorell, Werner, Simón, Varas, Calderón, Guzmán, Bono, San Martín, Eblen-Zajjur and Egaña.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histological evaluation. Biopsies were taken at days 7, 21, and 27 post-scaffold implantation. Histomorphology was evaluated with Hematoxylin-Eosin and Giemsa staining. Immunohistochemistry was performed to study the presence of macrophages (CD68) and endothelial cells (CD31). Colored arrows indicate: collagen fibers (black arrows), fibroblasts (white arrows), immune cells (red arrows), fibrin deposits (blue arrow), microalgae (green arrows), and vascular structures (yellow arrows). The indicated area in the left column of Hematoxylin-Eosin is magnified in the right column. Scale bars represent 100 μm for all pictures except for Hematoxylin-Eosin left column which represents 200 μm. All samples correspond to P8, except for Giemsa (P1).