REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
Escherichia coli, OP50	Caenorhabditis Genetics Center	OP50
Chemicals, peptides, and recombinant proteins
Sodium Chloride	MANAC	9317
Hipolypeptone (or any polypeptone)	WAKO	392-02115
Agar	TAISHO TECHNOS	17071902
Magnesium sulfate (MgSO4) heptahydrate	Sigma-Aldrich	19-0480-5
Calcium chloride (CaCl2)	WAKO	033-25035
Cholesterol	WAKO	034-03002
Ethanol	Sigma-Aldrich	64-17-5
Monopotassium phosphate (KH2PO4)	Sigma-Aldrich	24-5260-5
Dipotassium phosphate (K2HPO4)	Sigma-Aldrich	24-5240-5
Glycerol	Sigma-Aldrich	12-1120-5
LB Broth	Sigma-Aldrich	L3522-250G
M9 buffer	N/A	N/A
Fluorescein	Sigma-Aldrich	46955-1G-F
Gelatin	Nacalai Tesque	16631-05
Experimental models: Organisms/strains
C. elegans wild-type N2	Caenorhabditis Genetics Center	N2 (Bristol)
C. elegans N2; Ex[gcy-5p::gcamp6s; gcy-5p::mCherry; lin-44p::gfp]	Sato et al. (2021)	JN3500
C. elegans N2; Ex[npr-9p::gcamp6s; npr-9p::mCherry; lin-44p::gfp]	Sato et al. (2021)	JN3329
Software and algorithms
MetaMorph	Molecular Devices	MetaMorph Version7.7.4.0
Worm tracking software	Dr. Koichi Hashimoto/HAWKVISION	N/A
ImageJ	National Institutes of Health (NIH)	Java 1.8.0_172
Deposited data
The code for image analysis	Sato et al. (2021)	Mendeley Data: https://doi.org/10.17632/xcxngcy982.1
Other
Petri plates	IWAKI	1010-060
Terumo syringe, 50 mL	TERUMO	SS-50ESZ
Terumo syringe, 10 mL	TERUMO	SS-10SZ
Three-way stopcock	TERUMO	TS-TL2K
Polyethylene tube, inner diameter φ 0.58 mm	Imamura	01630-05
Stainless steel tube (cut 23G stainless steel tube into 1cm lengths)	N/A	N/A
Syringe pump	kd Scientific	78-8110
Peristaltic pump	ATTO corporation	SJ-1211
Optical microscope	OLYMPUS	BX51
Tracking stage (with control software)	HAWKVISION	HV-SAU02
EM-CCD Camera	HAMAMATSU	C9743-13 or successor model
Digital camera for infrared light imaging.	Point Grey	GRAS-03K2M-C
Glass chip 34 × 45 × 1.1	TOSHIN RIKO	N/A (Custom made, details to follow)
PDMS microfluidic chip (modified from Albrecht and Bargmann, 2011)	YODAKA	U944 (Custom made, details to follow)