. 2021 Dec 14;414(9):2841–2881. doi: 10.1007/s00216-021-03806-6

Table 1.

Amino acids essential for mAb binding to NA

Amino acid^a	NA subtype and the antigen (virus) used as immunogen^b
	N1		N2					N8	N9
	BR/07	CA/09	RI/57	TOK/67	MEM/98	MN/10	SGP/16	Dk/UK/63	Tern/AU/75	SH/13	AH/13
95 (95)		+^c
150			+						+
198					+
199					+			+
220					+				+
221				+	+	+
244										+
245							+
247 (248)	+	+					+				+
248 (249)	+					+
249 (250)	+
250											+
251											+
253				+							+
272 (273)	+	+
284								+
307 (308)	+	+
310 (311)		+
329			+						+
334			+								+
338										+
340 (338)	+	+								+
342 (339)	+	+	+	+				+		+
344 (341)	+
345											+
346 (343)	+
346								+
367 (364)		+						+	+
368			+	+					+		+
369									+
370			+						+		+
371											+
372 (369)		+						+	+
373											+
399 (396)	+
400 (397)	+	+						+	+		+
403			+
429						+				+
431										+
434											+
450 (449)		+
452 (451)		+
457 (456)	+
468							+

This table is an update of previously reported NA residues [54]

^aAmino acid residues follow N2 numbering with N1 numbering shown in parentheses

^bThe critical amino acid positions for antibody binding were originally reported for BR/07 [55], CA/09 [56–59], RI/57 [60], TOK/67 [61], MEM/98 [62], Dk/UK/63 [63], and Tern/AU/75 [64, 65], SH/13 [66], and AH/13 [67]

^c“+” indicates the amino acid position is essential for monoclonal antibody binding