Table 3.
LC–MS/MS analysis of the in-gel digestion of HeLa cell lysate TDP-43 band.
Peptide | Quantifier ion S/N | Qualifier ions (m/z) | % from ePARa |
---|---|---|---|
TDP56-79 | 40 | 882.3 | −15 |
981.8 | +15 | ||
TDP103-114 | 49 | 304.1 | +7 |
812.3 | +1 | ||
TDP152-160 | 18 | 767.4 | −11 |
604.3 | −13 | ||
TDP252-263 | 37 | 448.2 | −11 |
758.4 | −15 | ||
TDP276-293 | 22 | 376.3 | +7 |
694.3 | −24b |
Deviation from the expected peak area ratio for recTDP-43.
Failed acceptance criteria of ePAR within ±15%.