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. 2021 Dec 14;12(6):e03189-21. doi: 10.1128/mbio.03189-21

FIG 3.

FIG 3

Highly proliferative phenotype in MDSCs recruited to the lungs of RMs with ATB. Lung sections from ATB, LTBI, and naive groups were stained for CD66abce, CD33, and Ki67. Shown are the stitched confocal images (4-by-4 fields at ×10) of formalin-fixed and paraffin-embedded lung sections depicting proliferating PMN-MDSCs in control naive lung (A) and granuloma obtained from LTBI (B) and RMs with ATB (C). (D) Multilabel immunohistochemistry images exhibiting the distribution of proliferating PMN-MDSCs stained with CD66abce (green), CD33 (red), Ki67 (magenta), and DAPI (blue) in naive, LTBI, and ATB lung sections. (E) Graphical representation depicting a comparison of estimated fractions of Ki67+ PMN-MDSCs among the three groups. (F) Confocal images showing the distribution of proliferating PMN-MDSCs in various regions of a granuloma in ATB lung. (G) Estimated fraction of proliferating PMN-MDSCs (CD66abce+ CD33+ Ki67+) in the necrotic core (NC), intermediate myeloid layer (IM), lymphocytic cuff (LC), active lung interstitium (ALI), and naive lung interstitium (NLI). Statistical significance by one-way ANOVA with Tukey’s multiple-testing correction: *, P < 0.05; ****, P < 0.0001. Data represent mean ± SEM (n = 4).