FIG 7.

Candida species show variable macrophage lytic ability despite similar phagocytosis rates. (A) Percent lysis of macrophages by different Candida species. Macrophages were coincubated with Candida or medium only at a 3:1 MOI for 6 h. Lysis was measured by lactate dehydrogenase activity in the supernatant, with data shown as a percentage of activity of samples subjected to complete chemical lysis. *, P < 0.05, pairwise Student’s t test. Bars indicate the mean from three independent experiments, with error bars indicating the standard deviation. (B) Percentage of macrophages engulfing at least one Candida cell. Macrophages and Candida cells were coincubated at a 3:1 MOI for 1 h, before staining to identify internalized Candida cells as described in Materials and Methods. Representative images are shown in Fig. S5. *, P < 0.05, Wilcoxon signed-rank test. Bars indicate the mean from six independent experiments, with error bars indicating the range.