FIG 2.

Inductive production of 2-pyridones. (A) HPLC profiles showing the production of the compound peaks in different samples. Spores of M. robertsii (Mr), B. bassiana (Bb), and their mixtures at different ratios were inoculated into SDB for 9 days before metabolite extraction and profiling. (B) Phenotype of fungal (co)cultures. Spores of B. bassiana, M. robertsii, and their mixture (1:1) were inoculated into SDB for 9 days. (C) Upregulation of the tenS cluster genes in coculture (B. bassiana-M. robertsii at a 1:1 ratio). Tub, β-tubulin gene used as a reference. (D) Upregulation of the clustered genes by the overexpression of tenR but not the other putative transcription factor (BBA_07399). (E) HPLC analysis showing the production of compounds 1 to 7 by the overexpression of tenR. All cultures were grown in SDB for 9 days prior to metabolite extractions.