FIG 7.

ZipA mutations disrupt canonical and noncanonical cross-linking with FtsZ. Cell lysates of strain WM1074 carrying pUltra-pBpF, pKG110-FtsZ, and pDSW210-ZipA-GFP or derived constructs containing indicated mutations were assessed by Western blotting. (A to C) Canonical binding at ZipA M226X (A) or noncanonical binding at ZipA R314X (B) or ZipA E295X (C) was assessed by measuring CLS formation alone or in combination with the indicated canonical (purple) or noncanonical (black) binding mutations. Most noncanonical mutants preferentially reduced noncanonical cross-linking. Cross-linking efficiency relative to the control is indicated below +UV lanes and was normalized by total protein levels and ZipA-GFP expression for each strain. Each panel in panels A and B shows a composite of two blots.