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. Author manuscript; available in PMC: 2021 Dec 14.
Published in final edited form as: Cell Rep. 2021 Nov 2;37(5):109894. doi: 10.1016/j.celrep.2021.109894

Figure 2. Mapping of the interacting regions of SdhA and OCRL using solid-phase binding assays.

Figure 2.

(A) Constructs designed based on the coiled-coils prediction by ncoils (Lupas et al., 1991).

(B) SdhA-CBM2A binds to ASH-RhoGAP domain. Plate coated with ASH/RhoGAP was challenged with indicated SdhA constructs. EC50 was calculated as described (STAR Methods).

(C) High-affinity binding of CBM2A to ASH-RhoGAP. Protocol is as in (B).

(D) SdhA-CBM2 associates with ASH domain specifically. Plate coated with SdhA-CBM2 was challenged with His-tagged OCRL domains. Binding is detected by anti-His antibodies. His-Gαi was used as a negative control.

Linked to Table S3 and Figure S1. Data shown and error bars are means ± SD (n = 3 wells). Shown are representative assays of experiments performed in triplicate wells. Experiments were repeated three times.