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. Author manuscript; available in PMC: 2021 Dec 14.
Published in final edited form as: Cell Rep. 2021 Nov 2;37(5):109894. doi: 10.1016/j.celrep.2021.109894

Figure 7. OCRL-dependent accumulation of endosomal compartments on vacuole surrounding ΔsdhA mutant.

Figure 7.

(A) U937 macrophages were challenged for 3 h with L. pneumophila WT and ΔsdhA (MOI = 10). The presence of endosomal EEA1, retrograde trafficking cargo CIMPR, and recycling endosomal TfR on LCVs was evaluated by immunofluorescence microscopy in postnuclear supernatants of infected cells (STAR Methods). Scale bar represents 4 μm.

(B) Left: effect of siOCRL on protein expression in U937 cells. GAPDH was used as a loading control. Gel fractionated samples were immunoprobed with indicated antibodies. Right: the presence of EEA1 on LCVs was quantified from the infected U937 cell lysates as in (A). 50 vacuoles were quantified per condition from 3 separate infections. Data shown are mean ± SD (n = 3 biological replicate infections). Percent LCVs and percent EEA1-positive LCVs were compared within the groups using unpaired two-tailed Student’s t test. Statistical significance is represented as *p < 0.05 and **p < 0.01.