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Sample gel showing the correct assembly of pLASSO following multiple digestion with SwaI (lane 1; expected size 3204 bp), BamHI (lane 2; expected size 3204 bp), and BamHI + SwaI (lane 3; expected sizes of 1592 and 1631 bp, as one band at ~ 1.6 kb). Lane L: Quick-Load Purple 1 kb Plus DNA ladder. (Support Protocol 1).
