Table 21:
Negative control capture reaction components
| COMPONENT | AMOUNT | FINAL CONCENTRATION |
|---|---|---|
| LASSO probe M13 (1/100) | 1 μL | - |
| 10X Ampligase DNA Ligase Buffer | 1.5 μl | 1X |
| PCR grade water | Fill to 15 μl | - |
Negative control capture reaction components
| COMPONENT | AMOUNT | FINAL CONCENTRATION |
|---|---|---|
| LASSO probe M13 (1/100) | 1 μL | - |
| 10X Ampligase DNA Ligase Buffer | 1.5 μl | 1X |
| PCR grade water | Fill to 15 μl | - |