Table 22:
Library capture reaction components
| COMPONENT | AMOUNT | FINAL CONCENTRATION |
|---|---|---|
| Mature LASSO probe library | 10 ng | 0.7 ng/μL |
| DNA template | up to 2µg | 133 ng/μL |
| 10X Ampligase DNA Ligase Buffer | 1.5 μl | 1X |
| PCR grade water | Fill to 15 μl | - |
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