Skip to main content
. 2021 Dec 2;25(1):38. doi: 10.3892/mmr.2021.12554

Figure 2.

Figure 2.

Knockdown of MIR4435-2HG inhibits the proliferation, migration and invasion of liver cancer cells. (A) MIR4435-2HG expression was detected in liver cancer and normal liver tissues via RT-qPCR. ***P<0.001 vs. normal tissues. (B) MIR4435-2HG expression in normal liver cells and liver cancer cell lines. *P<0.05, **P<0.01 vs. THLE-2 cells. (C and D) After transfection with sh2HG-1, sh2HG-2, MIR4435-2HG or corresponding NCs, the expression levels of MIR4435-2HG were examined using RT-qPCR. *P<0.05, **P<0.01, ***P<0.001 vs. shNC, #P<0.05, ###P<0.001 vs. pcDNA3. (E) Proliferation was measured using the CCK-8 assay in liver cancer cells with indicated transfections. (F) Wound healing assay was used to assess cell migration (magnification, ×50). (G and H) The effects of MIR4435-2HG on cell migration and invasion were measured by Transwell assays (magnification, ×400). *P<0.05, **P<0.01 vs. shNC, #P<0.05, ##P<0.01 vs. pcDNA3. RT-qPCR, reverse transcription-quantitative PCR; sh, short hairpin RNA; sh2HG, shMIR4435-2HG; NC, negative control.