Skip to main content
. 2021 Dec 2;25(1):38. doi: 10.3892/mmr.2021.12554

Figure 5.

Figure 5.

MIR4435-2HG promotes B3GNT5 expression in liver cancer cells. (A) Dual-luciferase reporter assay was used to detect the binding between B3GNT5 and miR-136-5p. ***P<0.001 vs. miRNA NC. (B and C) mRNA and protein expression levels of B3GNT5 in liver cancer and adjacent normal tissues. **P<0.01 vs. normal tissues. (D and E) mRNA and protein expression levels of B3GNT5 in normal liver cell line and liver cancer cell lines. *P<0.05, **P<0.01 vs. THLE-2 cells. (F) MIR4435-2HG regulated B3GNT5 protein expression by targeting miR-136-5p, as shown following transfection with sh2HG-1 and miR-136-5p inhibitor. **P<0.01 vs. shNC + miR-NC inhibitor; #P<0.05, ##P<0.01 vs. sh2HG + miR-NC inhibitor. (G) MIR4435-2HG regulated B3GNT5 protein expression by targeting miR-136-5p, as shown following transfection with MIR4435-2HG and miR-136-5p mimics. **P<0.01 vs. pcDNA3 + miR-NC mimics; ##P<0.01 vs. MIR4435-2HG + miR-NC mimics. (H) Overexpression efficiency of B3GNT5. **P<0.01, ***P<0.001 vs. pcDNA3. (I-L) Knockdown of MIR4435-2HG significantly suppressed the proliferation, migration and invasion of liver cancer cells, these effects were reversed by the overexpression of B3GNT5. Magnification, ×50. *P<0.05, **P<0.01, ***P<0.001 vs. shNC + pcDNA3, #P<0.05, ##P<0.01, ###P<0.001 vs. sh2HG-1 + pcDNA3. (M) Data in the Gene Expression Profiling Interactive Analysis database demonstrated the positive relationship between B3GNT5 and MIR4435-2HG in liver cancer. miR/miRNA, microRNA; WT, wild-type; MUT, mutant; NC, negative control; sh, short hairpin RNA; sh2HG, shMIR4435-2HG.