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. 2021 Dec 14;11(12):202. doi: 10.1038/s41408-021-00594-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Peripheral blood and serum from 83 patients with hematological malignancies and 102 health care practitioners (HCP) were analyzed. B Antibody response at day 7 after the second-dose vaccination was measured using a CE-IVD serological SARS-CoV-2 multiplex bead-based flow cytometry immunoassay. It allows the simultaneous and quantitative detection of specific IgM, IgG, and IgA antibodies to four different antigens present in serum: (1) the receptor-binding domain (RBD) of the S-glycoprotein; (2) the stable trimer of the spicule (S) glycoprotein; (3) the nucleocapsid (N) protein; and (4) the main virus protease (Mpro). Detection of antibodies against the N and Mpro antigens allows the identification of individuals infected with SARS-CoV-2 prior or during vaccination. C Immune profiling of hematological patients and HCP prior vaccination was performed using multidimensional and computational flow cytometry. A total of 59 immune cell types were systematically measured in peripheral blood, including basophils, eosinophils, neutrophils, antigen-presenting cells (APC) and lymphocytes. D APC were sub-clustered into classical, intermediate, SLAN− and SLAN+ non-classical monocytes, as well as plasmacytoid and myeloid dendritic cells (pDC and mDC, respectively). E Sub-clustering of T cells into 32 subsets related to antigen-dependent differentiation, as well as activation and exhaustion phenotypes in helper and cytotoxic compartments. F Sub-clustering of B cells into 18 subsets related to antigen-dependent differentiation. CM, central memory; EM, effector memory; TEMRA, effector memory T cells re-expressing CD45RA; Tfh, follicular helper T cells; Treg, regulatory T cells; CPCs, circulating plasma cells.