Figure 4.

Effect of Astragaloside IV on PCNA expression. (A) PCNA expression was primarily detected in the pulmonary arteries (The black arrow refers to the positive expression of PCNA protein in pulmonary vascular tissue). Scale bar, 10 µm. Magnification, ×100. (B) Quantification of PCNA+ cells in lung sections. (C) PCNA mRNA expression in lung sections. PCNA protein expression levels in pulmonary artery smooth muscle cells were (D) determined via western blotting and (E) semi-quantified. *P<0.05; ^#P<0.01. PCNA, proliferating cell nuclear antigen; N, normoxia; H, hypoxia; T, treatment; DAPT, N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycinet-butyl ester.