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. 2021 Nov 28;25(1):30. doi: 10.3892/mmr.2021.12546

Figure 4.

Figure 4.

Restoration of MFAP3L expression reverses the miR-671-5p mimic-mediated effects on cancer cell viability, migration and invasion. (A) The protein levels of MFAP3L were determined in 95D and A549 cells following transfection with the MFAP3L overexpression plasmid or empty vector. (B) The protein expression levels of MFAP3L were detected in 95D and A549 cells co-transfected with either miR-NC or miR-671-5p mimics and MFAP3L or empty vector using western blot analysis. (C) The proliferative ability of co-transfected 95D and A549 cells was assessed by CCK-8 assay. (D) Wound healing assay was performed to assess the migratory ability of co-transfected 95D and A549 cells. (E and F) Transwell assay was performed to determine the invasive ability of co-transfected 95D and A549 cells. All experiments were performed in triplicate. **P<0.01 and ***P<0.001 vs. vector or miR-NC + vector; ###P<0.001 vs. miR-671-5p mimics + vector. MFAP3L, microfibril-associated protein 3-like; miR, microRNA; NC, negative control; CCK-8, Cell Counting Kit-8; OD, optical density.