Figure 3.

AS-DK143-NPs induces apoptosis in HeLa cells. Cells were treated with or without AS-DK143-NPs (10 and 30 µM) for 24 h. (A) Apoptotic cells were detected by TUNEL assay. Block arrows indicate the apoptotic cells. Scale bar=100 µM. (B) Nuclei were stained with Hoechst 33342 dye. Representative images were captured using confocal microscopy. Scale bar=50 µM. (C) mRNA-expression levels of p53, BAX and Bcl-2 evaluated by reverse transcription quantitative PCR. *P<0.05 vs. untreated group. Veh, vehicle; NPs, nanoparticles; AS, aspirin.