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A BPS-to-AG distance of 19 to 23 nt is optimal for step II. (A) Schematic of pre-mRNAs containing single AGs located at increasing distances from the BPS. (B) Pre-mRNAs were spliced for 45 min and analyzed on a 15% denaturing polyacrylamide gel. (C) Step II efficiency was calculated as the ratio of spliced products (mRNA and lariat) to splicing intermediates (lariat-exon and exon 1).
