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. 2021 Nov 30;4(6):1835–1848. doi: 10.1021/acsptsci.1c00224

Figure 7.

Figure 7

GPR35 activation suppresses LXR-induced lipid accumulation in hepatocytes from human GPR35a-HA expressing mice. Hepatocytes from GPR35a-HA expressing transgenic knock-in mice were used to assess changes in electrical impedance in response to lodoxamide (1 × 10–7 M) (A). Such signal was absent with co-addition of ML-145 (1 × 10–5M) (ML) (A). Hepatocytes (scale bar = 100 μm) isolated from these animals were maintained in culture and exposed to vehicle, T0901317 (8 × 10–6 M) (T090), T0901317/lodoxamide (5 × 10–6 M) (LOD), or T0901317/lodoxamide/ML-145 (1 × 10–5 M). (B) Representative visual images. Data quantified as relative lipid content (C). p < 0.05, a: versus vehicle, b: versus T0901317. (D) Effect of varying concentrations of lodoxamide is shown (EC50 = 1.7 ± 0.03 × 10–8 M). (E) ML-145 blocked the effect of lodoxamide in a concentration-dependent manner. p < 0.05, a: versus vehicle, b: versus T0901317, and c: versus lodoxamide.