Table 2. Mouse and Human GPR35 Primers Amplify Only the Appropriate Sequences and Confirm Expression of the Corresponding Sequence in Hepatocytes from Wild-Type and hGPR35-HA Micea.
| primers | WT | WT | h35 | h35 | KO | KO |
|---|---|---|---|---|---|---|
| mouse GPR35 | 30.2 | 30.8 | 36.8 | nd | nd | nd |
| 30.4 | 31.5 | 36.3 | 38.0 | nd | nd | |
| 30.1 | 30.7 | nd | nd | 37.5 | 39.0 | |
| human GPR35a-HA | nd | 38.4 | 35.6 | 33.5 | 37.4 | 37.0 |
| nd | 38.3 | 35.5 | 33.3 | 37.7 | 37.0 | |
| nd | 38.8 | 35.2 | 33.0 | 36.8 | 37.0 | |
| actin | 15.2 | 16.8 | 16.2 | 15.5 | 18.2 | 16.1 |
| 15.3 | 17.0 | 14.9 | 16.0 | 18.3 | 15.6 | |
| 15.2 | 16.7 | 16.1 | 15.2 | 17.7 | 15.8 |
a
Primers designed to selectively amplify cDNA corresponding to mGPR35 and hGPR35a-HA were used on samples prepared from hepatocytes isolated from wild-type (WT), hGPR35a-HA (h35), or GPR35 knock-out (KO) mice. Data from three technical replicates of two separate hepatocyte preparations are shown. Data are cycle numbers. nd = not detected. Actin was used as a house-keeping control gene.