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. 2021 Nov 30;12:779547. doi: 10.3389/fphys.2021.779547

Figure 2.

Figure 2

Effects of aging on protein synthesis during muscle denervation. Mice at 8months (8M) and 27months (27M) of age were subjected to denervation (right leg, DNV) and sham (contralateral left leg, CTL) surgeries, and TA and GAST muscles were collected 7days post-surgeries with puromycin injection. (A) Representative Western blots of TA samples. (B–D) Quantification of ubiquitinated proteins (B) and puromycin-labeled peptides per mg protein (C) or per muscle (D) in TA. (E) Representative Western blots of GAST samples. (F–H) Quantification of ubiquitinated proteins (F) and puromycin-labeled peptides per mg protein (G) or per muscle (H) in GAST. n=4–3 mice/group. Data are presented as mean±SEM. and expressed relative to 8M/CTL or as % of CTL (right panels in C,D,G,H). *p<0.05 and **p<0.01 vs. CTL by two-way mixed ANOVA. #p<0.05 vs. 8M within the same surgery groups by two-way mixed ANOVA. ††p<0.01 by unpaired t test. Inter, interaction between surgery effects (Surg) and age effects by two-way mixed ANOVA.