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. 2021 Nov 30;12:779547. doi: 10.3389/fphys.2021.779547

Figure 4.

Figure 4

Effects of aging on mammalian target of rapamycin complex 1 (mTORC1), ERK, and Akt signaling during muscle denervation. Mice at 8months (8M) and 27months (27M) of age were subjected to denervation (right leg, DNV) and sham (contralateral left leg, CTL) surgeries, and TA and GAST muscles were collected 7days post-surgeries. (A–C) Quantification of Western blots for phosphorylated (P)/total (T) ratios of p70S6K (A), S6 (B), and ERK (C) in TA. (D–F) Quantification of Western blots for phosphorylated (P)/total (T) ratios of p70S6K (D), S6 (E), and ERK (F) in GAST. (G,H) Quantification of Western blots for phosphorylated (P)/total (T) ratios of Akt (G) and glycogen synthase kinase-3β (GSK-3β; H) in TA. (I,J) Quantification of Western blots for phosphorylated (P)/total (T) ratios of Akt (I) and GSK-3β (J) in GAST. n=5–3 mice/group. Data are presented as mean±SEM. and expressed relative to 8M/CTL. *p<0.05 and **p<0.01 vs. CTL by two-way mixed ANOVA. #p<0.05 and ##p<0.01 vs. 8M within the same surgery groups by two-way mixed ANOVA. Inter, interaction between surgery effects (Surg) and age effects by two-way mixed ANOVA.