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. Author manuscript; available in PMC: 2022 Nov 24.
Published in final edited form as: Sci Transl Med. 2021 Nov 24;13(621):eabf7084. doi: 10.1126/scitranslmed.abf7084

Fig. 1. RAGE203, RAGE208, and RAGE229 bind to ctRAGE with nanomolar affinities.

Fig. 1.

Fluorescence titration of the compounds into the solution of ctRAGE resulted in the fluorescence quenching. The affinities of RAGE203 (A), RAGE208 (B), and RAGE229 (C) for the ctRAGE were estimated by fitting the data with the single-site binding isotherm and determined to be 30 ± 10, 24 ± 6, and 2 ± 1 nM, respectively. All experiments were performed in triplicate, and the results are expressed as the means ± SD.