FIG. 2.

Inactivation of Rat1p inhibits accumulation of CYH2 pre-mRNA decay intermediates in xrn1Δ cells. rat1-1 and rat1-1 xrn1Δ cells were grown at 24°C and then shifted to 37°C for 1, 2, or 4 h. Total RNA was isolated from each culture at the indicated time points and used for primer extension analysis of the 5′ ends (A) or Northern analysis of the levels of the CYH2 pre-mRNA (B), as in Fig. 1.