TABLE 3.

Effects of single or mutiple deletions of UPF1, NMD2, and UPF3 on the accumulation of total, capped, and decapped wild-type mRNAs^a

Strain	mRNA
	URA5			TCM1			PGK1
	Total	Capped	Uncapped	Total	Capped	Uncapped	Total	Capped	Uncapped
Wild type	1.00	0.84	0.16	1.00	0.66	0.34	1.00	0.95	0.05
upf1Δ	1.10	0.83	0.27	0.90	0.67	0.33	0.98	0.90	0.10
nmd2Δ	1.20	0.90	0.30	1.00	0.69	0.31	1.05	0.95	0.10
upf3Δ	1.20	0.84	0.36	0.96	0.70	0.26	0.95	0.90	0.05
xrn1Δ	2.50	0.38	2.12	2.10	0.30	1.80	1.70	0.95	0.75
xrn1Δ upf1Δ	2.40	0.72	1.68	1.80	0.50	1.30	1.40	0.76	0.64
xrn1Δ nmd2Δ	1.20	0.53	0.67	1.20	0.50	0.70	1.90	1.37	0.53
xrn1Δ upf3Δ	1.60	0.40	1.20	1.30	0.36	0.94	1.60	0.93	0.67
xrn1Δ upf1Δ nmd2Δ	2.50	0.75	1.75	1.70	0.50	1.20	1.40	0.76	0.64
xrn1Δ upf1Δ upf3Δ	2.40	0.72	1.68	1.70	0.50	1.20	1.40	0.81	0.59
xrn1Δ nmd2Δ upf3Δ	1.70	0.50	1.20	1.50	0.45	1.05	1.80	1.00	0.80
xrn1Δ upf1Δ nmd2Δ upf3Δ	2.35	0.70	1.65	1.70	0.50	1.20	1.40	0.81	0.59

^aThe Northern blot in Fig. 5 was used to measure the total levels of the URA5, TCM1, and PGK1 mRNAs in each sample. Northern blots in Fig. 6A and B were used to determine the percentage of capped and uncapped transcripts. The relative levels of total, capped, and decapped RNAs were calculated as explained in footnotes b and c to Table 2.