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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Oncogene. 2021 Jul 21;40(36):5455–5467. doi: 10.1038/s41388-021-01943-x

Figure 6.

Figure 6.

AMPK knockdown and metformin challenge affect the expression of genes encoding enzymes involved in the regulation of aminoacid utilization. PHGDH, PSAT1, PC and ASNS relative gene expression levels were measured by qRT-PCR. (a): MCF7ev and MCF7shAMPK cells challenged with 20mM Metformin. Metformin challenge had a significant effect on the expression of aminoacid utilization genes, while a more discrete effect of AMPK knockdown was observed. (b): MDA-MB231ev and MDA-MB231shAMPK cells challenged with 20mM Metformin. For MDA-MB231 cells silencing of AMPK had a more robust effect on PHGDH, PSAT1 and PC expression. All experiments are representative of 3 independent biological replicates. Data are presented as mean ± SEM. Statistical analysis was performed using One-way ANOVA. ns not significant; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.