Figure 2.

RNA variants and protein isoforms of the human GAPDH gene. An image copied from the NCBI database shows six RNA variants of GAPDH (top panel). The NR_152150.2 is annotated as a noncoding RNA, but, according to our analysis, it encodes an ORF for a GAPDH isoform of 161 AAs, shown as the shaded sequence with its ATG start codon and TAA stop codon boldfaced (middle panel). Because three of the five mRNA variants, that is, NM_0012589746.2, the NM_001289745.3, and the NM_002046.7, encode the same protein, the six RNAs encode a total of four protein isoforms, with their similarities and disparities as well as their numbers of AAs and molecular weights shown in the bottom panel.