FIG. 2.

Neu mutants induce Ets-2 transcriptional activation through threonine 72. The E18 luciferase reporter construct was cotransfected into EKO1 Ets-2-deficient cells with the various Neu add-back mutants, wild-type ets-2 expression vector (FNEts2) (open box), or mutant ets-2 with alanine substitution at threonine 72 (FNEts2 A72) (closed box). Also included was a β-actin-driven β-galactosidase expression vector, which served as an internal control for transfection efficiency. The ratio of luciferase activity relative to that observed for the empty pJ4 expression vector was used to calculate fold activation.