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. 2021 Dec 14;18:250. doi: 10.1186/s12985-021-01721-x

Fig. 5.

Fig. 5

Validation of RNA-seq profiles by real-time qPCR. To validate the RNA-seq data, the relative mRNA levels of 17 selected DEGs in the DnCPV_1 sample were examined by qPCR; The mRNA levels by qPCR are presented as the fold change compared with the Mock_1 sample after normalization against RPL13. The relative expression levels from the RNA-seq analysis were calculated as RPKM values. Error bars show mean ± SEM