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. 2021 Dec 14;19:508. doi: 10.1186/s12967-021-03173-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Down-regulated TRIM38 depended on GLUT1 to drive glycolytic process and tumor progression. a–c Cell viability was evaluated by Cell Counting Kit-8 (CCK-8) assay, in which GLUT1 knockdown suppressed the growth of TRIM38-deficient cells. d TRIM38 knockout enhanced T24 cells anchorage-independent growth in soft agar, which could be notably suppressed by TRIM38 overexpression (scale bars = 200 µm, left panel). Quantification of the soft agar colony formation assay results (right panel). e Knockout of TRIM38 significantly promoted glucose uptake, which could be restored by GLUT1 inhibition. f T24 cells (Ctrl & TRIM38-KO#1) with a Seahorse XF24 analyser for 100 min were used to depict the ECAR profiles. The metabolic inhibitors were injected sequentially at different time points as indicated. g EJ cells (Ctrl & TRIM38) with a Seahorse XF24 analyser for 100 min were utilized to show the ECAR profiles. The metabolic inhibitors were injected sequentially at different time points as indicated