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Differences in binding selectivity observed by native MS. (A) MS spectrum of apo-3CLpro during the titration assay. 3CLpro mainly presents in charge states P19+ and P18+. (B) MS spectrum of 3CLpro incubated with compound 2. The peaks of protein-ligand complexes were represented by P + L. (C) MS spectrum of 3CLpro incubated with compound 9-1. Nonspecific binding occurred.
