Table 1.
Substrate specificity
| Substrate | Relative activity |
|---|---|
| dThd | 100 |
| Trifluorothymidine (TFT) | 104.5 |
| Azidothymidine (AZT) | 55.0 |
| Fluorothymidine (FLT) | 43.5 |
| α-dThd | < 0.01 |
| Stavudine (D4T, 2′,3′.didehydro-2′,3′-dideoxythymidine) | 6.4 |
| dUrd | 106.1 |
| 5-fluorodeoxyuridine (5FdU) | 128.9 |
| 2′-deoxy-2′-fluoro-arabinofuranosyl-5-methyluracil (FMAU) | 82.6 |
| D-FMAU | 96.2 |
| L-FMAU | 34.6 |
| 2′-deoxy-2′-fluoro-arabinofuranosyl-5-iodouracil (FIAU) | 60.4 |
| Uridine | 1.7 |
| Cytidine | < 0.01 |
| dCyd | < 0.01 |
| dAdo | < 0.01 |
| dGuo | < 0.01 |
The assay was performed using a coupled spectrophotometric method at 21 °C. The substrate concentration was 0.1 mM with 1 mM ATP as the phosphate donor. The data are given as a percentage of that with dThd (0.95 μmol/mg/min)