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Phosphate donor specificity
| Phosphate donor | Relative activity |
|---|---|
| ATP | 100 |
| UTP | 19.1 |
| GTP | 23.9 |
| CTP | 13.7 |
| dATP | 70.7 |
| dTTP | 0.10 |
| dGTP | 13.9 |
| dCTP | 12.4 |
The assay was performed by using a radiochemical assay with [3H]-dThd as the substrate at 37 °C. The concentration of dThd was 0.1 mM, and the phosphate donor concentration was 1 mM. The data are given as a percentage of that with ATP as a phosphate donor (0.47 μmol/mg/min)
