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. 2021 Dec 14;18:59. doi: 10.1186/s12987-021-00294-9

Table 1.

Medium for neuronal expansion and differentiation

Reagent Supplier Catalogue number Final concentration
N2B27 medium
Neurobasal medium Gibco 21103049
N2 supplement Thermo Fisher 17502-048
B27 supplement Gibco 12587-010
Non-essential amino acids Gibco 11140050 1%
GlutaMAX™ supplement Gibco 35050038 1%
Penicillin-streptomycin Sigma P4333 1%
2-mercaptoethanol Gibco 31350-010 50 µM
Expansion medium
N2B27 medium
EGF Sigma E9644 10 ng/mL
FGF PeproTech 100-18B 10 ng/mL
Differentiation medium
N2B27 medium
BDNF PeproTech 450–02 20 ng/mL
GDNF PeproTech 450–10 10 ng/mL
Ascorbic acid Sigma A4544 100 µM
db-cAMP Sigma D0627 10 µM