Fig. 3.

In vitro targeting and lysosomal escape assay. a CLSM qualitative analysis and b flow cytometry offset of CT-26 cells treated with different liposomes. c DOX fluorescence intensity was quantified by flow cytometric. Scale bar, 50 μm. ^*P < 0.05, ^**P < 0.01. d The distribution of DOX loaded liposomes in CT-26 cells. DOX-Lip and DOX-R9-Lip were incubated with CT-26 cells for 0.5 h, 1 h, 2 h and 4 h. Green color: lysosomes. Blue color: cell nuclei. Red color: DOX. Scale bar, 10 μm