Fig. 2.

DFO and Fer-1 prevented ferroptotic cell death in IB3-1 cells. AECs were treated with FAC (100 µM) and erastin (Eras) (10 µM) in the presence or absence of DFO (100 µM) or Fer-1 (2 µM) for 12 h. Cell death kinetics were assessed by IncuCyte^® ZOOM system using SYTOX™ Green cell death marker. Representative images taken at 12 h post-treatment and cell death kinetics plot generated by normalising SYTOX positive cell count to starting cell confluence are shown (A). Cell viability was assessed by MTS assay (B) and cytotoxicity levels were assessed by measuring LDH release (C). Scale bar represents 300 µm. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001 for statistical analysis of the indicated groups