Fig. 2.

METTL3 overexpression impairs embryo attachment in vitro. a-b The expressions of METTL3, HOXA10, ITGB3 and EMX2 at the mRNA (a) and protein (b) levels in the METTL3-overexpressing Ishikawa cells were analyzed by qRT-PCR (a) and Western blotting(b), respectively. c The levels of m⁶A RNA methylation in the METTL3-overexpressing Ishikawa cells were evaluated by m⁶A RNA Methylation Assay Kit. d The levels of m⁶A in the METTL3-overexpressing Ishikawa cells were evaluated by dot blotting assay. e A vitro model of a confluent monolayer of Ishikawa cells co-cultured with BeWo spheroids was used to evaluate the embryo attachment. Bar 100 μm. The data are the average of three independent experiments (n=3). *P<0.05, **P<0.01 versus the indicated group